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RNAseq123

This is the released version of RNAseq123; for the devel version, see RNAseq123.

RNA-seq analysis is easy as 1-2-3 with limma, Glimma and edgeR


Bioconductor version: Release (3.23)

R package that supports the F1000Research workflow article on RNA-seq analysis using limma, Glimma and edgeR by Law et al. (2016).

Author: Charity Law, Monther Alhamdoosh, Shian Su, Xueyi Dong, Luyi Tian, Gordon Smyth and Matthew Ritchie

Maintainer: Matthew Ritchie <mritchie at wehi.edu.au>

Citation (from within R, enter citation("RNAseq123")):
Seminal Bioconductor project articles:

Huber W, Carey VJ, Gentleman R, Anders S, Carlson M, Carvalho BS, Bravo HC, Davis S, Gatto L, Girke T, Gottardo R, Hahne F, Hansen KD, Irizarry RA, Lawrence M, Love MI, MacDonald J, Obenchain V, Oleś AK, Pagès H, Reyes A, Shannon P, Smyth GK, Tenenbaum D, Waldron L, Morgan M (2015). "Orchestrating high-throughput genomic analysis with Bioconductor." Nature Methods, 12(2), 115–121. doi:10.1038/nmeth.3252.

Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JYH, Zhang J (2004). "Bioconductor: open software development for computational biology and bioinformatics." Genome Biology, 5(10), R80. doi:10.1186/gb-2004-5-10-r80.

Installation

To install this package, start R (version "4.6") and enter:


if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("RNAseq123")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("RNAseq123")
A guide to creating design matrices for gene expression experiments (English version) HTML R Script
RNA-seq analysis is easy as 1-2-3 with limma, Glimma and edgeR (Chinese version) HTML R Script
RNA-seq analysis is easy as 1-2-3 with limma, Glimma and edgeR (English version) HTML R Script

Details

biocViews GeneExpressionWorkflow, ImmunoOncologyWorkflow, Workflow
Version 1.36.0
License Artistic-2.0
Depends R (>= 3.3.0), Glimma(>= 1.1.9), limma, edgeR, gplots, RColorBrewer, Mus.musculus, R.utils, TeachingDemos, statmod, BiocWorkflowTools
Imports
System Requirements
URL https://f1000research.com/articles/5-1408/v3
See More
Suggests knitr, rmarkdown, BiocStyle
Linking To
Enhances
Depends On Me
Imports Me
Suggests Me
Links To Me

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package RNAseq123_1.36.0.tar.gz
Windows Binary (x86_64)
macOS Binary (big-sur-x86_64)
macOS Binary (sonoma-arm64)
Source Repository git clone https://git.bioconductor.org/packages/RNAseq123
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/RNAseq123
Package Short Url https://bioconductor.org/packages/RNAseq123/
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