# Thermal Shift Analysis in R

## Documentation for package ‘TSAR’ version 1.9.3

- DESCRIPTION file
- User guides, package vignettes and other documentation
- Package NEWS
- Package README

## Help Pages

analyze_norm | Analyze to Normalize
condition_IDs | TSAR Condition IDs
example_normalized_data | Example normalized TSA dataset
example_tsar_data | Example tsar_data file
gam_analysis | Analysis of all 96 wells through gam modeling
get_legend | Extract ggplot2 legend
graph_tsar | Graph tsar_data
join_well_info | Well information input function
merge_norm | Merge and format norm_data into tsar_data
merge_TSA | Merge TSA Raw Data and Analysis Files
model_beta | Beta-knot natural cubic spline model (Tm-centered via numerical_Tm)
model_boltzmann | Boltzmann Modeling on TSA data
model_fit | Refit and calculate derivative function
model_gam | Generalized Addidtive Modeling on TSA data
normalize | Normalize Fluorescence
normalize_fluorescence | Normalize Fluorescence Curve
numerical_Tm | Numerical Tm by treating Tm as inflection point
qPCR_data1 | qPCR_data1 Dataset
qPCR_data2 | qPCR_data2 Dataset
read_analysis | Read TSA Analysis Data
read_raw_data | Read TSA Raw Data
read_tsar | Read analysis result
remove_raw | Remove selected raw curves
rescale | Rescale values between minimum and maximum.
run_boltzmann | Run Boltzmann Modeling
screen | Screen raw curves
Tm_difference | Calculate Tm difference for all conditions
Tm_est | Find inflection point function
TSA_average | Average TSA Curves
TSA_boxplot | TSA Box Plot
TSA_compare_plot | Compare TSA curves to control
TSA_ligands | TSA Ligands
TSA_proteins | TSA Proteins
TSA_smoother_diagnostics | Diagnose Smoother Accuracy Across Conditions
TSA_Tms | Reformat TSA data into TSA Tms
TSA_wells_plot | TSA Well Curves Plot
view_deriv | View Derivative Curves
view_model | View Model
weed_raw | Weed raw data for corrupt curves
well_IDs | TSAR Well IDs
well_information | example well information Data
well_information_template | Well Information Template
write_tsar | write output files