Package: ddCt
Title: The ddCt Algorithm for the Analysis of Quantitative Real-Time
        PCR (qRT-PCR)
Version: 1.65.0
Date: 2021-07-30
Author: Jitao David Zhang, Rudolf Biczok, and Markus Ruschhaupt
Description: The Delta-Delta-Ct (ddCt) Algorithm is an approximation method to determine relative gene expression with quantitative real-time PCR (qRT-PCR) experiments. Compared to other approaches, it requires no standard curve for each primer-target pair, therefore reducing the working load and yet returning accurate enough results as long as the assumptions of the amplification efficiency hold. The ddCt package implements a pipeline to collect, analyse and visualize qRT-PCR results, for example those from TaqMan SDM software, mainly using the ddCt method. The pipeline can be either invoked by a script in command-line or through the API consisting of S4-Classes, methods and functions. 
Maintainer: Jitao David Zhang <jitao_david.zhang@roche.com>
biocViews: GeneExpression, DifferentialExpression,
        MicrotitrePlateAssay, qPCR
License: LGPL-3
Depends: R (>= 2.3.0), methods
Imports: Biobase (>= 1.10.0), RColorBrewer (>= 0.1-3), xtable, lattice,
        BiocGenerics
Collate: AllGlobals.R AllClasses.R AllGenerics.R AllFunctions.R
        AllMethods.R absoluteQ.R ddCtSplit.R output.R visualization.R
Suggests: testthat (>= 3.0.0), RUnit
Config/testthat/edition: 3
git_url: https://git.bioconductor.org/packages/ddCt
git_branch: devel
git_last_commit: 3ed4356
git_last_commit_date: 2025-04-15
Repository: Bioconductor 3.22
Date/Publication: 2025-06-04
NeedsCompilation: no
Packaged: 2025-06-04 22:26:29 UTC; biocbuild
Built: R 4.5.0; ; 2025-06-05 12:48:26 UTC; windows
