
@article{chang_complete_2015,
	title = {Complete {Genome} {Analysis} of a {PVYN}-{Wi} {Recombinant} {Isolate} from {Solanum} tuberosum in {China}},
	volume = {58},
	issn = {0014-3065, 1871-4528},
	url = {http://link.springer.com.eproxy1.lib.hku.hk/article/10.1007/s11540-015-9307-3},
	doi = {10.1007/s11540-015-9307-3},
	abstract = {The complete sequence of CF\_YL21, a Potato virus Y (PVY) isolate from Solanum tuberosum in China, was determined to be 9718 nucleotides in length, excluding the 3′-terminal poly(A) tail. The viral genome had a single open reading frame of 9186 nucleotides encoding a polyprotein of 3061 amino acids. The polyprotein was predicted to be cleaved into ten functional proteins by three viral proteases. Sequence analyses indicated that CF\_YL21 shared 97\% nucleotide identity with Wilga5 (PVYN-Wi), and two putative recombination signals were detected in the P1 and HC-Pro/P3 regions. Phylogenetic analyses, Bayesian Tip-association Significance (BaTS) test, and multiplex RT-PCR assay confirmed that the isolate had the similar molecular and genomic structure with PVYN-Wi, a PVY strain formed by recombination between PVYN and PVYO. To our knowledge, this is the first report of the complete sequence structure of PVYN-Wi strain from potato in China.},
	language = {en},
	number = {4},
	urldate = {2016-11-17},
	journal = {Potato Research},
	author = {Chang, Fei and Gao, Fangluan and Shen, Jianguo and Zou, Wenchao and Zhao, Shuang and Zhan, Jiasui},
	month = dec,
	year = {2015},
	pages = {377--389},
	file = {Full Text PDF:/Volumes/HOME/Zotero/storage/5GA9TM5W/Chang et al. - 2015 - Complete Genome Analysis of a PVYN-Wi Recombinant .pdf:application/pdf;Snapshot:/Volumes/HOME/Zotero/storage/UPZCGNCM/s11540-015-9307-3.html:text/html}
}