"Identifier","ID","Forward","Reverse","primer_length_fw","primer_length_rev","Direction","Degeneracy_fw","Degeneracy_rev","Run","DeltaH_fw","DeltaS_fw","Tm_C_fw","Tm_K_fw","DeltaH_rev","DeltaS_rev","Tm_C_rev","Tm_K_rev","melting_temp","melting_temp_diff","Basic_primer_coverage","Basic_Coverage_Ratio","Basic_Binding_Position_Start_fw","Basic_Binding_Position_End_fw","Basic_Binding_Position_Start_rev","Basic_Binding_Position_End_rev","Basic_Binding_Region_Allowed","Basic_Binding_Region_Allowed_fw","Basic_Binding_Region_Allowed_rev","Basic_Nbr_of_mismatches_fw","Basic_Nbr_of_mismatches_rev","Basic_Mismatch_pos_fw","Basic_Mismatch_pos_rev","Basic_Covered_Seqs","Basic_Relative_Forward_Binding_Position_Start_fw","Basic_Relative_Forward_Binding_Position_End_fw","Basic_Relative_Forward_Binding_Position_Start_rev","Basic_Relative_Forward_Binding_Position_End_rev","Basic_Relative_Reverse_Binding_Position_Start_fw","Basic_Relative_Reverse_Binding_Position_End_fw","Basic_Relative_Reverse_Binding_Position_Start_rev","Basic_Relative_Reverse_Binding_Position_End_rev","Basic_Off_primer_coverage","Basic_Off_Coverage_Ratio","Basic_Off_Binding_Position_Start_fw","Basic_Off_Binding_Position_End_fw","Basic_Off_Binding_Position_Start_rev","Basic_Off_Binding_Position_End_rev","Basic_Off_Binding_Region_Allowed","Basic_Off_Binding_Region_Allowed_fw","Basic_Off_Binding_Region_Allowed_rev","Basic_Off_Nbr_of_mismatches_fw","Basic_Off_Nbr_of_mismatches_rev","Basic_Off_Mismatch_pos_fw","Basic_Off_Mismatch_pos_rev","Basic_Off_Covered_Seqs","Basic_Off_Relative_Forward_Binding_Position_Start_fw","Basic_Off_Relative_Forward_Binding_Position_End_fw","Basic_Off_Relative_Forward_Binding_Position_Start_rev","Basic_Off_Relative_Forward_Binding_Position_End_rev","Basic_Off_Relative_Reverse_Binding_Position_Start_fw","Basic_Off_Relative_Reverse_Binding_Position_End_fw","Basic_Off_Relative_Reverse_Binding_Position_Start_rev","Basic_Off_Relative_Reverse_Binding_Position_End_rev","Basic_primer_specificity","primer_coverage","Coverage_Ratio","Binding_Position_Start_fw","Binding_Position_End_fw","Binding_Position_Start_rev","Binding_Position_End_rev","Binding_Region_Allowed","Binding_Region_Allowed_fw","Binding_Region_Allowed_rev","Nbr_of_mismatches_fw","Nbr_of_mismatches_rev","Mismatch_pos_fw","Mismatch_pos_rev","Covered_Seqs","Relative_Forward_Binding_Position_Start_fw","Relative_Forward_Binding_Position_End_fw","Relative_Forward_Binding_Position_Start_rev","Relative_Forward_Binding_Position_End_rev","Relative_Reverse_Binding_Position_Start_fw","Relative_Reverse_Binding_Position_End_fw","Relative_Reverse_Binding_Position_Start_rev","Relative_Reverse_Binding_Position_End_rev","Off_primer_coverage","Off_Coverage_Ratio","Off_Binding_Position_Start_fw","Off_Binding_Position_End_fw","Off_Binding_Position_Start_rev","Off_Binding_Position_End_rev","Off_Binding_Region_Allowed","Off_Binding_Region_Allowed_fw","Off_Binding_Region_Allowed_rev","Off_Nbr_of_mismatches_fw","Off_Nbr_of_mismatches_rev","Off_Mismatch_pos_fw","Off_Mismatch_pos_rev","Off_Covered_Seqs","Off_Relative_Forward_Binding_Position_Start_fw","Off_Relative_Forward_Binding_Position_End_fw","Off_Relative_Forward_Binding_Position_Start_rev","Off_Relative_Forward_Binding_Position_End_rev","Off_Relative_Reverse_Binding_Position_Start_fw","Off_Relative_Reverse_Binding_Position_End_fw","Off_Relative_Reverse_Binding_Position_Start_rev","Off_Relative_Reverse_Binding_Position_End_rev","primer_specificity","coverage_model","T_EVAL_coverage_model","T_off_EVAL_coverage_model","stop_codon","T_EVAL_stop_codon","substitution","T_EVAL_substitution","Structure_fw","Structure_rev","Structure_deltaG_fw","Structure_deltaG_rev","Structure_deltaG","Self_Dimer_DeltaG","Self_Dimer_Structure","Self_Dimer_Idx1","Self_Dimer_Idx2","Cross_Dimer_DeltaG","Cross_Dimer_Structure","Cross_Dimer_Idx1","Cross_Dimer_Idx2","gc_clamp_fw","gc_clamp_rev","gc_ratio_fw","gc_ratio_rev","no_runs_fw","no_runs_rev","no_repeats_fw","no_repeats_rev","EVAL_primer_length","EVAL_gc_clamp","EVAL_gc_ratio","EVAL_no_runs","EVAL_no_repeats","EVAL_melting_temp_range","EVAL_self_dimerization","EVAL_secondary_structure","EVAL_primer_coverage","EVAL_primer_specificity","EVAL_melting_temp_diff","EVAL_cross_dimerization","constraints_passed"
"1fw","Cardona1995|IGHV1-2*03|1_fw","accatggactgaagcttgaggatcctcttcttggtg","",36,0,"fw",1,1,"Cardona1995",-281330.783938815,-757.275334608031,65.95,339.1,NA,NA,NA,NA,65.95,6.61999999999999,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,"","","","","","","",".............(((.(((((...)))))..))).",NA,-0.06,0,-0.06,-4.204,"accatggactgaagcttgaggatcctcttcttggtg--------
                  ||||||||                  
        gtggttcttctcctaggagttcgaagtcaggtacca",1,1,-4.072,"                            accatggactgaagcttgaggatcctcttcttggtg
                             ||||||                             
gtcatcgtcctcgcaccttgtttcacacaggtacca----------------------------",1,2,2,0,0.5,0,2,0,2,0,FALSE,TRUE,TRUE,TRUE,TRUE,FALSE,TRUE,TRUE,FALSE,FALSE,FALSE,TRUE,FALSE
"2fw","Cardona1995|IGHV2-70*13|4_fw","accatggacacactttgttccacgctcctgctactg","",36,0,"fw",1,1,"Cardona1995",-288024.378585086,-771.861854684512,68.06,341.21,NA,NA,NA,NA,68.06,4.50999999999999,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,"","","","","","","","....................................",NA,0,0,0,-3.294,"                            accatggacacactttgttccacgctcctgctactg
                             ||||||                             
gtcatcgtcctcgcaccttgtttcacacaggtacca----------------------------",2,2,-4.072,"                            accatggactgaagcttgaggatcctcttcttggtg
                             ||||||                             
gtcatcgtcctcgcaccttgtttcacacaggtacca----------------------------",1,2,1,0,0.527777777777778,0,3,0,3,0,FALSE,TRUE,TRUE,TRUE,TRUE,FALSE,TRUE,TRUE,FALSE,FALSE,TRUE,TRUE,FALSE
"3fw","Cardona1995|IGHV3-72*01|5_fw","accatggagtttgggctgagctgggttttcc","",31,0,"fw",1,1,"Cardona1995",-243167.304015296,-648.879063097514,67.21,340.36,NA,NA,NA,NA,67.21,5.36,1,0.00657894736842105,"1","31","","","TRUE","TRUE","","3","","(2,3,5)","","83","-61","-31","","","325","295","","",0,0,"","","","","","","","","","","","","","","","","","","","",1,1,0.00657894736842105,"1","31","","","TRUE","TRUE","","3","","(2,3,5)","","83","-61","-31","","","325","295","","",0,0,"","","","","","","","","","","","","","","","","","","","",1,"0","TRUE","","0","TRUE","1","TRUE",".(((...(((....)))....))).......",NA,-0.39,0,-0.39,-3.294,"                       accatggagtttgggctgagctgggttttcc
                        ||||||                        
ccttttgggtcgagtcgggtttgaggtacca-----------------------",3,3,-4.072,"                       accatggacacactttgttccacgctcctgctactg
                        ||||||                             
ccttttgggtcgagtcgggtttgaggtacca----------------------------",2,3,2,0,0.548387096774194,0,4,0,2,0,FALSE,TRUE,TRUE,TRUE,TRUE,FALSE,TRUE,TRUE,TRUE,TRUE,FALSE,TRUE,FALSE
"4fw","Cardona1995|IGHV4-39*02|9_fw","aacatgaagcttctgtggttcttcctcctgctg","",33,0,"fw",1,1,"Cardona1995",-259451.7208413,-699.330783938815,64.16,337.31,NA,NA,NA,NA,64.16,8.41,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,"","","","","","","",".................................",NA,0,0,0,-2.737,"               aacatgaagcttctgtggttcttcctcctgctg
                     ||||||                     
gtcgtcctccttcttggtgtcttcgaagtacaa---------------",4,4,-2.703,"          accatggactgaagcttgaggatcctcttcttggtg
                     ||||||                   
gtcgtcctccttcttggtgtcttcgaagtacaa-------------",1,4,2,0,0.484848484848485,0,2,0,2,0,FALSE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,FALSE,FALSE,FALSE,TRUE,FALSE
"5fw","Cardona1995|IGHV5-a*02|10_fw","aagatggggtcaaccgccatcctcgccctcctc","",33,0,"fw",1,1,"Cardona1995",-263048.2791587,-695.633365200765,72.57,345.72,NA,NA,NA,NA,72.57,8.41,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,"","","","","","","","..(((((((....)).)))))............",NA,-2.17,0,-2.17,-0.361,"          aagatggggtcaaccgccatcctcgccctcctc
             ||||                          
ctcctcccgctcctaccgccaactggggtagaa----------",5,5,-2.42,"accatggactgaagcttgaggatcctcttcttggtg-----
                  |||||                  
        ctcctcccgctcctaccgccaactggggtagaa",1,5,2,0,0.636363636363636,0,4,0,2,0,FALSE,TRUE,FALSE,TRUE,TRUE,FALSE,TRUE,FALSE,FALSE,FALSE,FALSE,TRUE,FALSE
"6fw","Cardona1995|IGHV6_manual|11_fw","acaatgtaagcttccttcctcatcttcctgcccgtg","",36,0,"fw",1,1,"Cardona1995",-284128.10707457,-764.268642447419,66.3,339.45,NA,NA,NA,NA,66.3,6.27,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,"","","","","","","","....................................",NA,0,0,0,-2.02,"                acaatgtaagcttccttcctcatcttcctgcccgtg
                        ||||                        
gtgcccgtccttctactccttccttcgaatgtaaca----------------",6,6,-2.64,"                 aacatgaagcttctgtggttcttcctcctgctg
                       |||||                      
gtgcccgtccttctactccttccttcgaatgtaaca--------------",4,6,3,0,0.5,0,3,0,1,0,FALSE,TRUE,TRUE,TRUE,TRUE,FALSE,TRUE,TRUE,FALSE,FALSE,FALSE,TRUE,FALSE
