<design_settings>
<constraint_set>
<name>Input_Constraints</name>
<constraint>
<name>cross_dimerization</name>
<values>min=-7</values>
</constraint>
<constraint>
<name>melting_temp_diff</name>
<values>min=0,max=5</values>
</constraint>
<constraint>
<name>secondary_structure</name>
<values>min=-1</values>
</constraint>
<constraint>
<name>primer_coverage</name>
<values>min=1</values>
</constraint>
<constraint>
<name>primer_length</name>
<values>min=18,max=22</values>
</constraint>
<constraint>
<name>primer_specificity</name>
<values>min=1,max=1</values>
</constraint>
<constraint>
<name>gc_clamp</name>
<values>min=1,max=3</values>
</constraint>
<constraint>
<name>gc_ratio</name>
<values>min=0.4,max=0.6</values>
</constraint>
<constraint>
<name>no_runs</name>
<values>min=0,max=4</values>
</constraint>
<constraint>
<name>no_repeats</name>
<values>min=0,max=4</values>
</constraint>
<constraint>
<name>self_dimerization</name>
<values>min=-5</values>
</constraint>
<constraint>
<name>melting_temp_range</name>
<values>min=55,max=65</values>
</constraint>
</constraint_set>
<constraint_set>
<name>Input_Constraint_Boundaries</name>
<constraint>
<name>cross_dimerization</name>
<values>min=-9</values>
</constraint>
<constraint>
<name>melting_temp_diff</name>
<values>min=0,max=7.5</values>
</constraint>
<constraint>
<name>secondary_structure</name>
<values>min=-2</values>
</constraint>
<constraint>
<name>primer_coverage</name>
<values>min=1</values>
</constraint>
<constraint>
<name>primer_length</name>
<values>min=18,max=22</values>
</constraint>
<constraint>
<name>primer_specificity</name>
<values>min=0.8,max=1</values>
</constraint>
<constraint>
<name>gc_clamp</name>
<values>min=0,max=4</values>
</constraint>
<constraint>
<name>gc_ratio</name>
<values>min=0.3,max=0.7</values>
</constraint>
<constraint>
<name>no_runs</name>
<values>min=0,max=6</values>
</constraint>
<constraint>
<name>no_repeats</name>
<values>min=0,max=6</values>
</constraint>
<constraint>
<name>self_dimerization</name>
<values>min=-7</values>
</constraint>
<constraint>
<name>melting_temp_range</name>
<values>min=45,max=75</values>
</constraint>
</constraint_set>
<constraint_set>
<name>Coverage_Constraints</name>
<constraint>
<name>coverage_model</name>
<values>max=0.05</values>
</constraint>
<constraint>
<name>stop_codon</name>
<values>min=0,max=1</values>
</constraint>
<constraint>
<name>substitution</name>
<values>min=0,max=1</values>
</constraint>
</constraint_set>
<constraint_set>
<name>PCR_conditions</name>
<constraint>
<name>use_taq_polymerase</name>
<values>active</values>
</constraint>
<constraint>
<name>Na_concentration</name>
<values>0</values>
</constraint>
<constraint>
<name>Mg_concentration</name>
<values>1.5</values>
</constraint>
<constraint>
<name>K_concentration</name>
<values>50</values>
</constraint>
<constraint>
<name>Tris_concentration</name>
<values>0</values>
</constraint>
<constraint>
<name>primer_concentration</name>
<values>200</values>
</constraint>
<constraint>
<name>template_concentration</name>
<values>0.0128</values>
</constraint>
<constraint>
<name>cycles</name>
<values>25</values>
</constraint>
</constraint_set>
<constraint_set>
<name>constraint_settings</name>
<constraint>
<name>allowed_mismatches</name>
<values>7</values>
</constraint>
<constraint>
<name>allowed_other_binding_ratio</name>
<values>1</values>
</constraint>
<constraint>
<name>allowed_region_definition</name>
<values>within</values>
</constraint>
</constraint_set>
</design_settings>
