"Identifier","ID","Forward","Reverse","primer_length_fw","primer_length_rev","Direction","Degeneracy_fw","Degeneracy_rev","Run","DeltaH_fw","DeltaS_fw","Tm_C_fw","Tm_K_fw","DeltaH_rev","DeltaS_rev","Tm_C_rev","Tm_K_rev","melting_temp","melting_temp_diff","Basic_primer_coverage","Basic_Coverage_Ratio","Basic_Binding_Position_Start_fw","Basic_Binding_Position_End_fw","Basic_Binding_Position_Start_rev","Basic_Binding_Position_End_rev","Basic_Binding_Region_Allowed","Basic_Binding_Region_Allowed_fw","Basic_Binding_Region_Allowed_rev","Basic_Nbr_of_mismatches_fw","Basic_Nbr_of_mismatches_rev","Basic_Mismatch_pos_fw","Basic_Mismatch_pos_rev","Basic_Covered_Seqs","Basic_Relative_Forward_Binding_Position_Start_fw","Basic_Relative_Forward_Binding_Position_End_fw","Basic_Relative_Forward_Binding_Position_Start_rev","Basic_Relative_Forward_Binding_Position_End_rev","Basic_Relative_Reverse_Binding_Position_Start_fw","Basic_Relative_Reverse_Binding_Position_End_fw","Basic_Relative_Reverse_Binding_Position_Start_rev","Basic_Relative_Reverse_Binding_Position_End_rev","Basic_Off_primer_coverage","Basic_Off_Coverage_Ratio","Basic_Off_Binding_Position_Start_fw","Basic_Off_Binding_Position_End_fw","Basic_Off_Binding_Position_Start_rev","Basic_Off_Binding_Position_End_rev","Basic_Off_Binding_Region_Allowed","Basic_Off_Binding_Region_Allowed_fw","Basic_Off_Binding_Region_Allowed_rev","Basic_Off_Nbr_of_mismatches_fw","Basic_Off_Nbr_of_mismatches_rev","Basic_Off_Mismatch_pos_fw","Basic_Off_Mismatch_pos_rev","Basic_Off_Covered_Seqs","Basic_Off_Relative_Forward_Binding_Position_Start_fw","Basic_Off_Relative_Forward_Binding_Position_End_fw","Basic_Off_Relative_Forward_Binding_Position_Start_rev","Basic_Off_Relative_Forward_Binding_Position_End_rev","Basic_Off_Relative_Reverse_Binding_Position_Start_fw","Basic_Off_Relative_Reverse_Binding_Position_End_fw","Basic_Off_Relative_Reverse_Binding_Position_Start_rev","Basic_Off_Relative_Reverse_Binding_Position_End_rev","Basic_primer_specificity","primer_coverage","Coverage_Ratio","Binding_Position_Start_fw","Binding_Position_End_fw","Binding_Position_Start_rev","Binding_Position_End_rev","Binding_Region_Allowed","Binding_Region_Allowed_fw","Binding_Region_Allowed_rev","Nbr_of_mismatches_fw","Nbr_of_mismatches_rev","Mismatch_pos_fw","Mismatch_pos_rev","Covered_Seqs","Relative_Forward_Binding_Position_Start_fw","Relative_Forward_Binding_Position_End_fw","Relative_Forward_Binding_Position_Start_rev","Relative_Forward_Binding_Position_End_rev","Relative_Reverse_Binding_Position_Start_fw","Relative_Reverse_Binding_Position_End_fw","Relative_Reverse_Binding_Position_Start_rev","Relative_Reverse_Binding_Position_End_rev","Off_primer_coverage","Off_Coverage_Ratio","Off_Binding_Position_Start_fw","Off_Binding_Position_End_fw","Off_Binding_Position_Start_rev","Off_Binding_Position_End_rev","Off_Binding_Region_Allowed","Off_Binding_Region_Allowed_fw","Off_Binding_Region_Allowed_rev","Off_Nbr_of_mismatches_fw","Off_Nbr_of_mismatches_rev","Off_Mismatch_pos_fw","Off_Mismatch_pos_rev","Off_Covered_Seqs","Off_Relative_Forward_Binding_Position_Start_fw","Off_Relative_Forward_Binding_Position_End_fw","Off_Relative_Forward_Binding_Position_Start_rev","Off_Relative_Forward_Binding_Position_End_rev","Off_Relative_Reverse_Binding_Position_Start_fw","Off_Relative_Reverse_Binding_Position_End_fw","Off_Relative_Reverse_Binding_Position_Start_rev","Off_Relative_Reverse_Binding_Position_End_rev","primer_specificity","coverage_model","T_EVAL_coverage_model","T_off_EVAL_coverage_model","stop_codon","T_EVAL_stop_codon","substitution","T_EVAL_substitution","Structure_fw","Structure_rev","Structure_deltaG_fw","Structure_deltaG_rev","Structure_deltaG","Self_Dimer_DeltaG","Self_Dimer_Structure","Self_Dimer_Idx1","Self_Dimer_Idx2","Cross_Dimer_DeltaG","Cross_Dimer_Structure","Cross_Dimer_Idx1","Cross_Dimer_Idx2","gc_clamp_fw","gc_clamp_rev","gc_ratio_fw","gc_ratio_rev","no_runs_fw","no_runs_rev","no_repeats_fw","no_repeats_rev","EVAL_primer_length","EVAL_gc_clamp","EVAL_gc_ratio","EVAL_no_runs","EVAL_no_repeats","EVAL_melting_temp_range","EVAL_self_dimerization","EVAL_secondary_structure","EVAL_primer_coverage","EVAL_primer_specificity","EVAL_melting_temp_diff","EVAL_cross_dimerization","constraints_passed"
"1fw","Padyukov2001|IGKV2D-29*01|1_fw","tatttgggttcatttaaagag","",21,0,"fw",1,1,"Padyukov2001",-154951.7208413,-431.087476099426,42.93,316.08,NA,NA,NA,NA,42.93,13.46,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,"","","","","","","",".....................",NA,0,0,0,-1.798,"tatttgggttcatttaaagag---------
            ||||||            
         gagaaatttacttgggtttat",1,1,-2.519,"tatttgggttcatttaaagag
      |||            
acgtcaccacaccggta----",1,2,1,0,0.285714285714286,0,3,0,2,0,TRUE,TRUE,FALSE,TRUE,TRUE,FALSE,TRUE,TRUE,FALSE,FALSE,FALSE,TRUE,FALSE
"2fw","Padyukov2001|IGKV2-29*01|2_fw","atggccacaccactgca","",17,0,"fw",1,1,"Padyukov2001",-130075.525812619,-344.770554493308,56.39,329.54,NA,NA,NA,NA,56.39,13.46,24,0.387096774193548,"10,146,146,327,321,10,146,140,161,158,158,161,1,1,1,1,1,1,1,1,1,1,9,1","26,162,162,343,337,26,162,156,177,174,174,177,17,17,17,17,17,17,17,17,17,17,25,17","","","TRUE,FALSE,FALSE,FALSE,FALSE,TRUE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE","TRUE,FALSE,FALSE,FALSE,FALSE,TRUE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE","","7,7,7,7,7,7,7,7,7,7,7,7,7,7,6,5,6,6,6,6,6,5,7,7","","(5,7,9,10,12,14,17),(2,6,7,8,10,13,17),(2,6,7,8,10,13,17),(1,4,6,10,11,13,15),(1,4,6,10,11,13,15),(5,7,9,10,12,14,17),(2,6,7,8,10,13,17),(2,6,7,8,10,13,17),(5,6,12,13,15,16,17),(5,6,12,13,15,16,17),(5,6,12,13,15,16,17),(5,6,12,13,15,16,17),(5,6,7,9,13,14,15),(5,6,7,9,13,14,15),(5,6,7,9,13,14),(5,6,9,13,14),(5,6,7,9,13,14),(5,6,7,9,13,14),(5,6,7,9,13,14),(5,6,7,9,13,14),(5,6,7,9,13,14),(5,6,9,13,14),(5,6,7,9,13,14,15),(4,6,7,10,14,15,17)","","2,3,4,15,16,21,22,23,38,39,40,45,46,47,48,49,50,51,53,54,55,56,58,64","-58,78,78,259,259,-58,78,78,99,96,96,99,-61,-61,-61,-61,-61,-61,-61,-61,-61,-61,-62,-58","-42,94,94,275,275,-42,94,94,115,112,112,115,-45,-45,-45,-45,-45,-45,-45,-45,-45,-45,-46,-42","","","313,177,177,-4,-4,313,177,177,174,174,174,174,316,316,316,319,316,316,316,316,316,319,320,313","297,161,161,-20,-20,297,161,161,158,158,158,158,300,300,300,303,300,300,300,300,300,303,304,297","","",10,0.161290322580645,"146,146,327,321,146,140,161,158,158,161","162,162,343,337,162,156,177,174,174,177","","","FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE","FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE","","7,7,7,7,7,7,7,7,7,7","","(2,6,7,8,10,13,17),(2,6,7,8,10,13,17),(1,4,6,10,11,13,15),(1,4,6,10,11,13,15),(2,6,7,8,10,13,17),(2,6,7,8,10,13,17),(5,6,12,13,15,16,17),(5,6,12,13,15,16,17),(5,6,12,13,15,16,17),(5,6,12,13,15,16,17)","","3,4,15,16,22,23,38,39,40,45","78,78,259,259,78,78,99,96,96,99","94,94,275,275,94,94,115,112,112,115","","","177,177,-4,-4,177,177,174,174,174,174","161,161,-20,-20,161,161,158,158,158,158","","",0.583333333333333,0,0,"","","","","","","","","","","","","","","","","","","","",10,0.161290322580645,"","","","","","","","","","","","","","","","","","","","",0,"","FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE","FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE","","TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE","","TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE",".................",NA,0,0,0,-4.574,"         atggccacaccactgca
          ||||||          
acgtcaccacaccggta---------",2,2,-2.519,"tatttgggttcatttaaagag
      |||            
acgtcaccacaccggta----",1,2,0,0,0.588235294117647,0,2,0,2,0,FALSE,FALSE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,FALSE,FALSE,FALSE,TRUE,FALSE
