"Identifier","ID","Forward","Reverse","primer_length_fw","primer_length_rev","Direction","Degeneracy_fw","Degeneracy_rev","Run","DeltaH_fw","DeltaS_fw","Tm_C_fw","Tm_K_fw","DeltaH_rev","DeltaS_rev","Tm_C_rev","Tm_K_rev","melting_temp","melting_temp_diff","Basic_primer_coverage","Basic_Coverage_Ratio","Basic_Binding_Position_Start_fw","Basic_Binding_Position_End_fw","Basic_Binding_Position_Start_rev","Basic_Binding_Position_End_rev","Basic_Binding_Region_Allowed","Basic_Binding_Region_Allowed_fw","Basic_Binding_Region_Allowed_rev","Basic_Nbr_of_mismatches_fw","Basic_Nbr_of_mismatches_rev","Basic_Mismatch_pos_fw","Basic_Mismatch_pos_rev","Basic_Covered_Seqs","Basic_Relative_Forward_Binding_Position_Start_fw","Basic_Relative_Forward_Binding_Position_End_fw","Basic_Relative_Forward_Binding_Position_Start_rev","Basic_Relative_Forward_Binding_Position_End_rev","Basic_Relative_Reverse_Binding_Position_Start_fw","Basic_Relative_Reverse_Binding_Position_End_fw","Basic_Relative_Reverse_Binding_Position_Start_rev","Basic_Relative_Reverse_Binding_Position_End_rev","Basic_Off_primer_coverage","Basic_Off_Coverage_Ratio","Basic_Off_Binding_Position_Start_fw","Basic_Off_Binding_Position_End_fw","Basic_Off_Binding_Position_Start_rev","Basic_Off_Binding_Position_End_rev","Basic_Off_Binding_Region_Allowed","Basic_Off_Binding_Region_Allowed_fw","Basic_Off_Binding_Region_Allowed_rev","Basic_Off_Nbr_of_mismatches_fw","Basic_Off_Nbr_of_mismatches_rev","Basic_Off_Mismatch_pos_fw","Basic_Off_Mismatch_pos_rev","Basic_Off_Covered_Seqs","Basic_Off_Relative_Forward_Binding_Position_Start_fw","Basic_Off_Relative_Forward_Binding_Position_End_fw","Basic_Off_Relative_Forward_Binding_Position_Start_rev","Basic_Off_Relative_Forward_Binding_Position_End_rev","Basic_Off_Relative_Reverse_Binding_Position_Start_fw","Basic_Off_Relative_Reverse_Binding_Position_End_fw","Basic_Off_Relative_Reverse_Binding_Position_Start_rev","Basic_Off_Relative_Reverse_Binding_Position_End_rev","Basic_primer_specificity","primer_coverage","Coverage_Ratio","Binding_Position_Start_fw","Binding_Position_End_fw","Binding_Position_Start_rev","Binding_Position_End_rev","Binding_Region_Allowed","Binding_Region_Allowed_fw","Binding_Region_Allowed_rev","Nbr_of_mismatches_fw","Nbr_of_mismatches_rev","Mismatch_pos_fw","Mismatch_pos_rev","Covered_Seqs","Relative_Forward_Binding_Position_Start_fw","Relative_Forward_Binding_Position_End_fw","Relative_Forward_Binding_Position_Start_rev","Relative_Forward_Binding_Position_End_rev","Relative_Reverse_Binding_Position_Start_fw","Relative_Reverse_Binding_Position_End_fw","Relative_Reverse_Binding_Position_Start_rev","Relative_Reverse_Binding_Position_End_rev","Off_primer_coverage","Off_Coverage_Ratio","Off_Binding_Position_Start_fw","Off_Binding_Position_End_fw","Off_Binding_Position_Start_rev","Off_Binding_Position_End_rev","Off_Binding_Region_Allowed","Off_Binding_Region_Allowed_fw","Off_Binding_Region_Allowed_rev","Off_Nbr_of_mismatches_fw","Off_Nbr_of_mismatches_rev","Off_Mismatch_pos_fw","Off_Mismatch_pos_rev","Off_Covered_Seqs","Off_Relative_Forward_Binding_Position_Start_fw","Off_Relative_Forward_Binding_Position_End_fw","Off_Relative_Forward_Binding_Position_Start_rev","Off_Relative_Forward_Binding_Position_End_rev","Off_Relative_Reverse_Binding_Position_Start_fw","Off_Relative_Reverse_Binding_Position_End_fw","Off_Relative_Reverse_Binding_Position_Start_rev","Off_Relative_Reverse_Binding_Position_End_rev","primer_specificity","coverage_model","T_EVAL_coverage_model","T_off_EVAL_coverage_model","stop_codon","T_EVAL_stop_codon","substitution","T_EVAL_substitution","Structure_fw","Structure_rev","Structure_deltaG_fw","Structure_deltaG_rev","Structure_deltaG","Self_Dimer_DeltaG","Self_Dimer_Structure","Self_Dimer_Idx1","Self_Dimer_Idx2","Cross_Dimer_DeltaG","Cross_Dimer_Structure","Cross_Dimer_Idx1","Cross_Dimer_Idx2","gc_clamp_fw","gc_clamp_rev","gc_ratio_fw","gc_ratio_rev","no_runs_fw","no_runs_rev","no_repeats_fw","no_repeats_rev","EVAL_primer_length","EVAL_gc_clamp","EVAL_gc_ratio","EVAL_no_runs","EVAL_no_repeats","EVAL_melting_temp_range","EVAL_self_dimerization","EVAL_secondary_structure","EVAL_primer_coverage","EVAL_primer_specificity","EVAL_melting_temp_diff","EVAL_cross_dimerization","constraints_passed"
"1fw","Moraes2003|IGLV5-39*02|1_fw","ggaattcaagcttctgcagatggcctggactcctctc","",37,0,"fw",1,1,"Moraes2003",-293818.833652008,-788.644837476099,68.02,341.17,NA,NA,NA,NA,68.02,3.95,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,"","","","","","","","(((.((((.(((.........))).)))).)))....",NA,-1.54,0,-1.54,-2.946,"     ggaattcaagcttctgcagatggcctggactcctctc
                  ||||||                  
ctctcctcaggtccggtagacgtcttcgaacttaagg-----",1,1,-3.208,"            ggaattcaagcttctgcagatggcctggactcctctc
                  |||||||                        
tttctctcctcagggtccgttcgaaccccc-------------------",1,3,1,0,0.540540540540541,0,2,0,2,0,FALSE,TRUE,TRUE,TRUE,TRUE,FALSE,TRUE,FALSE,FALSE,FALSE,TRUE,TRUE,FALSE
"2fw","Moraes2003|IGLV1-41*01|2_fw","gctcccctctcctcctcaccc","",21,0,"fw",1,1,"Moraes2003",-162344.646271511,-429.988049713193,64.07,337.22,NA,NA,NA,NA,64.07,4.71000000000001,30,0.857142857142857,"8,8,8,8,8,11,8,8,8,8,8,8,8,8,8,8,8,8,8,8,8,8,8,26,8,8,8,8,8,8","28,28,28,28,28,31,28,28,28,28,28,28,28,28,28,28,28,28,28,28,28,28,28,46,28,28,28,28,28,28","","","TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE","TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE","","3,3,1,6,7,7,6,7,7,6,7,4,3,6,6,6,6,7,6,6,5,6,6,5,6,7,7,6,4,4","","(2,5,20),(2,5,20),(4),(2,3,5,7,8,17),(2,3,5,7,8,11,20),(1,2,3,4,5,7,8),(2,3,5,7,8,11),(2,3,5,7,8,11,20),(2,3,5,7,8,11,20),(2,3,5,7,8,11),(2,3,4,12,18,19,21),(2,3,14,18),(2,3,19),(2,3,12,13,14,20),(2,3,6,7,18,19),(2,3,6,7,18,19),(2,3,6,7,18,19),(2,3,6,8,14,18,20),(2,3,4,11,14,18),(2,3,4,11,14,18),(2,3,4,14,18),(2,3,6,14,17,19),(2,3,6,14,17,19),(1,4,18,19,21),(2,3,5,18,19,20),(2,3,5,18,19,20,21),(2,3,5,18,19,20,21),(2,3,5,8,11,14),(2,3,5,11),(2,3,5,11)","","1,2,3,4,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,26,28,29,30,31,35,36","-51,-51,-45,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-51,-39,-51,-51,-51,-51,-51,-51","-31,-31,-25,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-31,-19,-31,-31,-31,-31,-31,-31","","","318,318,309,315,316,316,316,317,316,316,304,304,309,309,309,309,309,307,309,301,303,309,303,306,331,331,331,315,336,336","298,298,289,295,296,296,296,297,296,296,284,284,289,289,289,289,289,287,289,281,283,289,283,286,311,311,311,295,316,316","","",2,0.0571428571428571,"79,79","99,99","","","FALSE,FALSE","FALSE,FALSE","","7,7","","(1,4,12,13,15,18,21),(1,4,12,13,15,18,21)","","29,30","20,20","40,40","","","260,260","240,240","","",0.9375,11,0.314285714285714,"8,8,8,11,8,8,8,8,8,8,8","28,28,28,31,28,28,28,28,28,28,28","","","TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE","TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE","","3,3,1,7,6,6,4,3,5,4,4","","(2,5,20),(2,5,20),(4),(1,2,3,4,5,7,8),(2,3,5,7,8,11),(2,3,5,7,8,11),(2,3,14,18),(2,3,19),(2,3,4,14,18),(2,3,5,11),(2,3,5,11)","","1,2,3,7,8,11,13,14,22,35,36","-51,-51,-45,-51,-51,-51,-51,-51,-51,-51,-51","-31,-31,-25,-31,-31,-31,-31,-31,-31,-31,-31","","","318,318,309,316,316,316,304,309,303,336,336","298,298,289,296,296,296,284,289,283,316,316","","",2,0.0571428571428571,"","","","","","","","","","","","","","","","","","","","",1,"0.053030303030303,0.053030303030303,0,0.0136363636363636,0.0484848484848485,0.0484848484848485,0.0484848484848485,0.0318181818181818,0.053030303030303,0.0227272727272727,0.0227272727272727","TRUE,TRUE,TRUE,FALSE,FALSE,TRUE,TRUE,FALSE,FALSE,TRUE,FALSE,TRUE,TRUE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,TRUE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,FALSE,TRUE,TRUE","FALSE,FALSE","0,0,0,0,0,0,0,0,0,1,1","TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE","1,1,1,1,1,1,1,1,1,1,1","TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE",".....................",NA,0,0,0,0.888,"                   gctcccctctcctcctcaccc
                   ||                   
cccactcctcctctcccctcg-------------------",2,2,-1.372,"gctcccctctcctcctcaccc---------------
                  |||               
      tttctctcctcagggtccgttcgaaccccc",2,3,3,0,0.714285714285714,0,4,0,2,0,TRUE,TRUE,FALSE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,TRUE,FALSE
"3fw","Moraes2003|IGLV7-43|3_fw","cccccaagcttgcctgggactcctctcttt","",30,0,"fw",1,1,"Moraes2003",-233876.195028681,-621.204588910134,68.78,341.93,NA,NA,NA,NA,68.78,4.71000000000001,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,0,0,"","","","","","","","","","","","","","","","","","","","",0,0,"","","","","","","","","","","","","","","","","","","","",0,"","","","","","","","..((((........))))............",NA,-2.4,0,-2.4,-1.986,"              cccccaagcttgcctgggactcctctcttt
                  ||||||||                  
tttctctcctcagggtccgttcgaaccccc--------------",3,3,-3.208,"            ggaattcaagcttctgcagatggcctggactcctctc
                  |||||||                        
tttctctcctcagggtccgttcgaaccccc-------------------",1,3,0,0,0.6,0,5,0,3,0,FALSE,FALSE,TRUE,FALSE,TRUE,FALSE,TRUE,FALSE,FALSE,FALSE,TRUE,TRUE,FALSE
