\name{FlowSorted.Blood.450k}
\alias{FlowSorted.Blood.450k}
\title{
  Illumina Human Methylation data from 450k on sorted blood cell
  populations
}
\description{
  This function downloads (once; cached locally afterwards via
  \link[BiocFileCache:BiocFileCache-class]{BiocFileCache}) and returns
  an \code{RGset} containing Illumina 450k DNA methylation measurements
  on 60 samples from Reinius et al. 2012, which can be used by the
  \link{minfi} package to estimate cellular composition from whole blood
  samples. This data may also be useful to individuals as example
  Illumina 450k data for trying preprocessing methods across a variety
  of Bioconductor packages.

  As of package version 1.51.2, this data is no longer bundled directly
  with the package; it is hosted on Zenodo (\doi{10.5281/zenodo.21996983})
  and downloaded on first use.
}

\usage{
FlowSorted.Blood.450k
}

\details{ The \code{FlowSorted.Blood.450k} object is based on samples
  assayed as part of Reinius et al (2012). Please cite this paper, if
  the data is used.  If you're using this data together with the
  \link{minfi} package, please see the package vignette for details on
  how to cite that package.

  A script for obtaining this dataset is available in the \code{scripts}
  directory of this package.
}
\value{
  An object of class \code{RGChannelSet}.
}
\references{
  Reinius, L. E. et al.
  \emph{Differential DNA Methylation in Purified Human Blood Cells:
    Implications for Cell Lineage and Studies on Disease
    Susceptibility}.
  PLoS ONE (2012), 7:e41361.
  \doi{10.1371/journal.pone.0041361}

  Houseman E.A. et al
  \emph{DNA methylation arrays as surrogate measures of cell mixture
    distribution.}
  BMC Bioinformatics (2012), 13:p86.
  \doi{10.1186/1471-2105-13-86}
  
  Jaffe A.E. and Irizarry R.A.
  \emph{Accounting for cellular heterogeneity is critical in epigenome-wide association studies}
  Genome Biology (2013), 15:R31.
  \doi{10.1186/gb-2014-15-2-r31}
}
\seealso{ \code{\link{FlowSorted.Blood.450k.JaffeModelPars}} and
  \code{\link{FlowSorted.Blood.450k.compTable}} for additional datasets
  derived from this one. See the minfi package for 
  tools for estimating cell type composition in blood using these data..}
\examples{
\donttest{
library(FlowSorted.Blood.450k)
rgset <- FlowSorted.Blood.450k
}
}
\keyword{datasets}
