scPipe
This is the development version of scPipe; for the stable release version, see scPipe.
Pipeline for single cell multi-omic data pre-processing
Bioconductor version: Development (3.24)
A preprocessing pipeline for single cell RNA-seq/ATAC-seq data that starts from the fastq files and produces a feature count matrix with associated quality control information. It can process fastq data generated by CEL-seq, MARS-seq, Drop-seq, Chromium 10x and SMART-seq protocols.
Author: Luyi Tian [aut], Shian Su [aut, cre], Shalin Naik [ctb], Shani Amarasinghe [aut], Oliver Voogd [aut], Phil Yang [aut], Matthew Ritchie [ctb]
Maintainer: Shian Su <su.s at wehi.edu.au>
citation("scPipe")):
Huber W, Carey VJ, Gentleman R, Anders S, Carlson M, Carvalho BS, Bravo HC, Davis S, Gatto L, Girke T, Gottardo R, Hahne F, Hansen KD, Irizarry RA, Lawrence M, Love MI, MacDonald J, Obenchain V, Oleś AK, Pagès H, Reyes A, Shannon P, Smyth GK, Tenenbaum D, Waldron L, Morgan M (2015). "Orchestrating high-throughput genomic analysis with Bioconductor." Nature Methods, 12(2), 115–121. doi:10.1038/nmeth.3252.
Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, Dudoit S, Ellis B, Gautier L, Ge Y, Gentry J, Hornik K, Hothorn T, Huber W, Iacus S, Irizarry R, Leisch F, Li C, Maechler M, Rossini AJ, Sawitzki G, Smith C, Smyth G, Tierney L, Yang JYH, Zhang J (2004). "Bioconductor: open software development for computational biology and bioinformatics." Genome Biology, 5(10), R80. doi:10.1186/gb-2004-5-10-r80.
Installation
To install this package, start R (version "4.6") and enter:
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
## The following initializes the development version of Bioconductor
BiocManager::install(version = "devel")
BiocManager::install("scPipe")
For older versions of R, please refer to the appropriate Bioconductor release.
Documentation
To view documentation for the version of this package installed in your system, start R and enter:
browseVignettes("scPipe")| scPipe: a flexible data preprocessing pipeline for 3' end scRNA-seq data | HTML | R Script |
| scPipe: a flexible data preprocessing pipeline for scATAC-seq data | HTML | R Script |
| Reference Manual | ||
| NEWS | Text |
Details
| biocViews | DataImport, GeneExpression, GenomeAnnotation, ImmunoOncology, Preprocessing, QualityControl, RNASeq, SequenceMatching, Sequencing, SingleCell, Software, Visualization |
| Version | 2.13.1 |
| In Bioconductor since | BioC 3.6 (R-3.4) (9 years) |
| License | GPL (>= 2) |
| Depends | R (>= 4.2.0), SingleCellExperiment |
| Imports | AnnotationDbi, basilisk, BiocGenerics, biomaRt, Biostrings, data.table, dplyr, DropletUtils, flexmix, GenomicRanges, GenomicAlignments, GGally, ggplot2, glue (>= 1.3.0), grDevices, graphics, hash, IRanges, magrittr, MASS, Matrix (>= 1.5.0), mclust, methods, MultiAssayExperiment, org.Hs.eg.db, org.Mm.eg.db, purrr, Rcpp (>= 0.11.3), reshape, reticulate, Rhtslib, rlang, robustbase, Rsamtools, Rsubread, rtracklayer, SummarizedExperiment, S4Vectors, scales, stats, stringr, tibble, tidyr, tools, utils, vctrs (>= 0.5.2) |
| System Requirements | C++11, GNU make |
| URL | https://github.com/LuyiTian/scPipe |
| Bug Reports | https://github.com/LuyiTian/scPipe |
See More
| Suggests | BiocStyle, DT, GenomicFeatures, grid, igraph, kableExtra, knitr, locStra, plotly, rmarkdown, RColorBrewer, readr, reshape2, RANN, shiny, scater(>= 1.11.0), testthat, xml2, umap |
| Linking To | Rcpp, Rhtslib(>= 1.13.1), testthat |
| Enhances | |
| Depends On Me | |
| Imports Me | stPipe |
| Suggests Me | |
| Links To Me | |
| Build Report | Build Report |
Package Archives
Follow Installation instructions to use this package in your R session.
| Source Package | scPipe_2.13.1.tar.gz |
| Windows Binary (x86_64) | scPipe_2.13.1.zip (64-bit only) |
| macOS Binary (big-sur-x86_64) | scPipe_2.13.1.tgz |
| macOS Binary (sonoma-arm64) | scPipe_2.13.1.tgz |
| Source Repository | git clone https://git.bioconductor.org/packages/scPipe |
| Source Repository (Developer Access) | git clone git@git.bioconductor.org:packages/scPipe |
| Bioc Package Browser | https://code.bioconductor.org/browse/scPipe/ |
| Package Short Url | https://bioconductor.org/packages/scPipe/ |
| Package Downloads Report | Download Stats |